Figure 5
From: Regulation of TGF-β signalling by Fbxo11, the gene mutated in the Jeff otitis media mouse mutant
Lung phenotype. a. Sections through the lungs of E15.5 wild-type (WT) and homozygote (Jf/Jf) embryos, immunohistochemically stained with antibodies against Ki67, Smad2 and pSmad2 (scale bars 200 μm and 20 μm as indicated). Graphs: comparison of the percentage of cells positive for Ki67, Smad2 and pSmad2 in proximal airways. b. Western blot analysis: cytoplasmic and nuclear fractions from wild-type (wt), heterozygote (Jf/+) and homozygote (Jf/Jf) whole E15.5 lungs. Equal amounts of protein were subjected to tris-acetate PAGE, transferred and probed with pSmad2 and actin antibody for loading control. Graph: comparison of the normalised pSmad2 signals in the two fractions. c. Haematoxylin-eosin stained sections through new born wild-type (WT) and homozygote (Jf/Jf) lungs. Scale bars 200 μm. Graph: comparison of the number of airways for three regions of 5.3 × 106 μm2 taken at random and the width of 3 × 30 airways for each genotype. P-values were determined using two-tailed T-test.