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Figure 4 | PathoGenetics

Figure 4

From: Regulation of TGF-β signalling by Fbxo11, the gene mutated in the Jeff otitis media mouse mutant

Figure 4

Immunolocalization in eyelid tissue. a. Coronal sections through the eye of E16 wild-type (WT) and homozygote (Jf/Jf) embryos immunohistochemically stained with antibodies against Fbox11, TGF-β3, TGFβR-I, Smad3, Smad2 and Smad4. Scale bar 200 μm. Graph: comparison of the percentage of epithelial cells positive for Smad2 in E16 upper and lower eyelids. b. Coronal sections through the eyes of E15.5 (before fusion) and E16 (after fusion) in wild-type (WT) and homozygote (Jf/Jf) embryos stained with pSmad2 antibody (scale bar 200 μm). Epidermis (ep), basal cells (bc) and dermis (d). Graph: comparison of the percentage of epithelial cells with positive nuclear and cytoplasmic localization of pSmad2 in E16 upper and lower eyelids. c. Coronal sections through the eyes of E16 in wild-type (WT) and homozygote (Jf/Jf) embryos stained with Ki67 and cleaved caspase-3 antibodies. Scale bar 200 μm. Graph: comparison of the percentage of epithelial cell positive for Ki67 and cleaved caspase-3 in E16 upper and lower eyelids. P-values were determined using two-tailed T-test comparing each homozygote eyelid with the wild type.

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