Figure 6From: Abnormal autophagy, ubiquitination, inflammation and apoptosis are dependent upon lysosomal storage and are useful biomarkers of mucopolysaccharidosis VIReduction of autophagic, ubiquitination, and mitochondrial anomalies in livers of MPS VI rats following arylsulfatase B gene transfer and DS normalization. (A) Representative western blot analysis of tissue lysates from livers of 6-month-old NR and MPS VI rats treated (TR) or not (AF) with AAV. Membranes were blotted with anti-BCN1, -LC3, -ubiquitin, -p62, -COX IV and -actin antibodies. (B) Quantification of western blots. Values are expressed as fold of increase compared with NR rat tissues (NR = 1) and are the mean of three independent experiments. *P ≤ 0.05, **P ≤ 0.01.Back to article page