Figure 2From: Abnormal autophagy, ubiquitination, inflammation and apoptosis are dependent upon lysosomal storage and are useful biomarkers of mucopolysaccharidosis VIGlycosaminoglycan (GAG) accumulation in visceral organs of MPS VI rats. (A) Toluidine blue staining of semi-thin sections (1 μm thick) shows vacuoles filled with storage (arrows) in lysosomes of cells from liver, spleen, and kidney of 6-month-old MPS VI affected (AF) rats, which were absent in the same tissues of normal (NR) rats. Magnification: 100×. (B) Quantitative measurement of GAG accumulation via dimethyl-methylene blue method on tissue lysates from liver, spleen, and kidney of AF and NR rats. (GAG levels are expressed as μg per mg of proteins: μg GAG/mg prot). *P ≤ 0.01.Back to article page