Figure 5

Leptomycin B treatment of cells transfected with a construct expressing either wild-type or mutant HYLS1. Average percentages of cells showing either cytoplasmic (white, cells showing cytoplasmic staining only) or nuclear (gray, cells showing only nuclear or nuclear and cytoplasmic staining) localization are given. After leptomycin B treatment (LM+), both wild-type (wt) and mutant (mut) HYLS1 are showing nuclear localization that significantly differs from the untreated state (LM-) (both wt and mutant p < 0.001). The proportion of nuclear localization in untreated mutant cells versus wt cells is also significant (p < 0.001).