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Figure 4 | PathoGenetics

Figure 4

From: Pancreatic islet expression profiling in diabetes-prone C57BLKS/J mice reveals transcriptional differences contributed by DBA loci, including Plagl1 and Nnt

Figure 4

Nicotinamide nucleotide transhydrogenase is derived from the DBA strain in BLKS mice. (A) Sequencing of exon 1 of the Nnt gene in BLKS and B6 mice confirms the DBA origin of the gene in the BLKS genome. B6 mice possess the codon for threonine (Thr) at residue 35, whereas the BLKS mice have the codon for methionine (Met). (B) RT-PCR analysis of the Nnt RNA in B6 and BLKS. A smaller RT-PCR fragment is amplified from B6 RNA (743 bp) than BLKS RNA (1798 bp), confirming the deletion of exons 8 to 11 previously described in B6 mice [32]. The two smaller bands (bracket) in B6 mice are likely mis-splicing artifacts from downstream exons which are flanked by the PCR primers. M – marker.

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PathoGenetics

ISSN: 1755-8417