Table 1 Primers and UPL probes used in the real-time reverse-transcriptase polymerase chain reaction experiments
From: High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi
Transcript | Forward | Reverse | Probe |
|---|---|---|---|
Oct4 | GTTGGAGAAGGTGGAACCAA | CTCCTTCTGCAGGGCTTTC | 95 |
β-actin | AAGGCCAACCGTGAAAAGAT | GTGGTACGACCAGAGGCATAC | 56 |
Gata4 | GGAAGACACCCCAATCTCG | CATGGCCCCACAATTGAC | 13 |
Fgf5 | AAAACCTGGTGCACCCTAGA | CATCACATTCCCGAATTAAGC | 29 |
Brachyury | CGACCACAAAGATGTAATGGAG | CCAGCACCAGGAACAAGC | 66 |
Hand1 | AGCGGAAAAGGGAGTTGC | GCGCCCTTTAATCCTCTTCT | 51 |
Cdx2 | CTGCCACACTTGGGCTCT | CTTGGCTCTGCGGTTCTG | 34 |
Human β-catenin | GCAGAGTGCTGAAGGTGCTA | TCTGTCAGGTGAAGTCCTAAAGC | 31 |
Mouse β-catenin | ATGAAGGCGTGGCAACATAC | TGTGGCTTGTCCTCAGACAT | 56 |