Figure 4
From: High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi
Oct4 knock-down using pRosa26-DEST phenocopies the conventional knock-out phenotype. (a) Western blot of undifferentiated embryonic stem (ES) cells transiently transfected with pcDNA6.2-GW/EmGFP-miR lacZ and pcDNA6.2-GW/EmGFP-miR Oct4. (b) Southern blot confirmation of two independent MG-4 derived ES clones targeted with the pRosa26-miOct4 construct. The screening strategy is the same as in Figure 2a before expression of Cre. (c) Oct4 expression after tamoxifen treatment to activate the CreERT2 in MG-4 cells and its derivates. (d) Marker analysis of Oct-4 knock-down ES cells.