Figure 5

qPCR validation of the expression profiling results. Quantitative PCR analysis of a selection of six genes differentially regulated in Smad4^+/E6sadcells. Gene expression was quantified in normal intestinal tissues from two wild-type (WT) and three Smad4^+/E6sad(Smad4) animals and is plotted as ratio over the reference gene (see Materials and Methods). Each bar represents the average of three independent experiments.