Figure 8From: Mutations in the nuclear localization sequence of the Aristaless related homeobox; sequestration of mutant ARX with IPO13 disrupts normal subcellular distribution of the transcription factor and retards cell divisionIncreased proportion of mitotic cells transfected with nuclear localization sequence (NLS) mutant ARX. (A) The percentage of HEK293T cells transfected and (B) the percentage of mitotic cells transfected with Myc-ARX-Wt or NLS mutant constructs for 24, 48 and 72 h was determined from between 1200 and 4000 cells per sample, from at least three separate transfection reactions. Full-length Myc-tagged constructs transfected are indicated in the legend ARX Wt (open), R332P (vertical light grey stripes), T333N (grey), R379L (horizontal dark grey) and R379S (dark grey) with the time post-transfection indicated on the X-axis.Back to article page